Application of gold nanoparticles for improvement of analytical characteristics of conductometric enzyme biosensors

Abstract In the work, possibility of using nanoparticles gold (AuNPs) to upgrade bioselective elements biosensors in order improve their analytical characteristics is considered. The based on acetylcholinesterase (AChE), butyryl cholinesterase (BuChE) and glucose oxidase (GOD) were used as an experimental model. Immobilization enzymes surfaces conductometric transducers was performed by crosslinking corresponding glutaraldehyde. conditions immobilization AChE with optimized. Thus, we determined optimal values concentration agent (glutaraldehyde), duration immobilization, enzyme AuNPs ratio, size. performance investigated compared only. It also examined how addition element affects biosensor stability. particular, reproducibility preparation continuous operation tested well stability at storage. shown that presence composition can some biosensors, which may be promising for further study use.